Coding
fliC_MCS

Part:BBa_K1617000:Design

Designed by: Johann Bauerfeind   Group: iGEM15_Berlin   (2015-09-15)


Modular Flagellin - FliC_MCS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 628
    Illegal BamHI site found at 577
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 583
    Illegal AgeI site found at 634
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. Modularity of D3 Domain

2. Substitution of Methionins

3. Removing restriction sites

4. Compatibility of Multi-Cloning-Site

Source

Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655) Gene ID: 949101 Synonyms: ECK1922; flaF; H; hag; JW1908

References