Coding
fliC_MCS
Part:BBa_K1617000:Design
Designed by: Johann Bauerfeind Group: iGEM15_Berlin (2015-09-15)
Modular Flagellin - FliC_MCS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 628
Illegal BamHI site found at 577 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 583
Illegal AgeI site found at 634 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
1. Modularity of D3 Domain
2. Substitution of Methionins
3. Removing restriction sites
4. Compatibility of Multi-Cloning-Site
Source
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655) Gene ID: 949101 Synonyms: ECK1922; flaF; H; hag; JW1908